Modulation of apoptosis against P. falciparum by low dose radiation in human PBMCs.
نویسندگان
چکیده
BACKGROUND Implications of low dose radiation (LDR) have been well reported in cancer therapy but data is scanty on the therapeutic application of LDR in infectious diseases. METHODOLOGY Human peripheral blood mononuclear cells (PBMCs) were cultured and exposed to 0.07 Gy. P. falciparum infected RBCs were mixed with the PBMCs after five hours of irradiation. Thereafter, PBMCs were monitored for micronuclei and apoptosis. RESULTS The low dose pre-irradiated PBMCs which were subsequently challenged with parasite, showed a reduction in micronuclei frequency and apoptosis as compared to controls. CONCLUSION LDR inhibited apoptosis against P. falciparum in human PBMCs.
منابع مشابه
Effect of famotidine on radiation induced apoptosis in human peripheral blood leukocytes
ABSTRACTBackground: Radioprotective effects of famotidine, an antagonist of H2 receptor clinically used for peptic ulcer treatment, was previously shown on radiation-induced micronuclei and chromosomal aberration in human peripheral blood lymphocytes and mouse bone marrow cells. This study was conducted to investigate radioprotective property of famotidine against radiation induced apoptosis i...
متن کاملQuantitation of genome damage and transcriptional profile of DNA damage response genes in human peripheral blood mononuclear cells exposed in vitro to low doses of neutron radiation
Background: Humans are exposed to ionizing radiation from different sources that include natural, occupational, medical, accidental exposures. Evaluation of the effect of low level of neutron exposure to human cells in vitro has important implications to human health. Attempts were made to measure genome damage, transcriptional profile of DNA damage response and repair genes in peripheral blood...
متن کاملIntermittent low dose irradiation enhances the effectiveness of radio-therapy for human breast adenocarcinoma cell line MDA–MB–231
Introduction: Hormesis and adaptive responses are two important biological effects of low-dose ionizing radiation (LDIR) in organism and mammalian cell lines. Notably, LDIR generates distinct biological effects in cancer cells from normal cells, e.g., it may affect the growth of cancer cells via the activation of certain cell signaling pathway, which does not exist in normal ...
متن کاملStudy of radio protective effect of Thymus vulgaris essential oil against ionizing radiation on peripheral blood mononuclear cells (PBMCs)
Introduction: Today ionizing radiation (IR) plays an important role in medicine, both in diagnostic and therapeutic field. Doctors, medical staff and patients are all in danger of side effects of IR so there is a need to protect them against deleterious effects of ionizing radiation. Thymus vulgaris has biological activities like antioxidant and antibacterial activity. Aim of...
متن کاملLow kilovolt “prospective ECG-triggering” vs. “retrospective ECG-gating” coronary CTA: comparison of image quality and radiation dose
Background: To compare image quality and radiation doses of low kilovolt (kV) “prospective ECG-triggering” (PT) and standard “retrospective ECG-gating” (RG) coronary computed tomography (CT) angiography. Materials and Methods: A total of 101 consecutive patients (76 males, 25 females; mean age: 55.44 ± 8.28 years) with low-to-intermediate risk status for coronary artery disease and with a body ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of infection in developing countries
دوره 3 11 شماره
صفحات -
تاریخ انتشار 2009